鸭Ccna1启动子克隆、序列分析及在鸭骨骼肌中的发育性表达

doi:10.11843/j.issn.0366-6964.2017.02.022

Abstract

Abstract:

To investigate the roles of Ccna1 gene in duck skeletal muscle development, its promoter sequence was amplified by RT-PCR, then was analyzed using bioinformatics methods. qRT-PCR was used to detect the expression profiles of Ccna1 during duck embryonic muscle development, as well as the expression profiles of myogenic transcription factors predicted to take part in regulation of Ccna1 transcription. We obtained 2 213 bp promoter region of duck Ccna1 gene. Bioinformatics analysis showed there were typical TATA-box, CAAT-box and binding sites for transcription factors MyoD, MRF4, MyoG, Sp1, PITX1 and MEF2 in the Ccna1 gene promoter region. The qRT-PCR data showed that MyoD, MRF4, MyoG and Ccna1 expressed in duck muscle tissues at embryonic development stages. In the breast muscle, the expression of MRF4 was the highest in E23 and was significantly higher than that in D8 (P<0.05). In the leg muscle, the expression of MRF4 and MyoG were the highest in E17, and was significantly higher than that in D2, D8 (P<0.05). Clustering results showed that the expression patterns of Ccna1 was consistent with MyoD in breast muscle, while in the leg muscle, Ccna1 was clustered together with MRF4 and MyoG. It was preliminary concluded that Ccna1 was involved in the duck skeletal muscle development, and Ccna1 might be associated with the MRF4 and MyoG during the leg muscle development, whereas in the breast muscle development, its expression might be regulated by MyoD.

CLC Number:

S834
S813.1

YANG Zhen, LI Yan-ying, LUO Jun, ZHANG Tao, LIU He-he, PU Mei, XIAO Xiang-jun, REN Yan-lin. Molecular Cloning, Bioinformatics Analysis of the Duck Ccna1 Gene Promoter Region，and Its Differential Expression Profiles in Skeletal Muscle Tissues[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(2): 377-384.

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Molecular Cloning, Bioinformatics Analysis of the Duck Ccna1 Gene Promoter Region，and Its Differential Expression Profiles in Skeletal Muscle Tissues

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